A team of Massachusetts General Hospital (MGH) investigators has made the first steps towards development of bioartificial replacement limbs suitable for transplantation.  In their report, which has been published online in the journal Biomaterials, the researchers describe using an experimental approach previously used to build bioartificial organs to engineer rat forelimbs with functioning vascular and muscle tissue.  They also provided evidence that the same approach could be applied to the limbs of primates. “The composite nature of our limbs makes building a functional biological replacement particularly challenging,” explained Harald Ott, MD, of the MGH Department of Surgery and the Center for Regenerative Medicine, senior author of the paper.  “Limbs contain muscles, bone, cartilage, blood vessels, tendons, ligaments and nerves – each of which have to be rebuilt and require a specific supporting structure called the matrix.  We have shown that we can maintain the matrix of all of these tissues in their natural relationships to each other, that we can culture the entire construct over prolonged periods of time, and that we can repopulate the vascular system and musculature.” The authors note that more than 1.5 million individuals in the U.S. have lost a limb, and although prosthetic technology has greatly advanced, the devices still have many limitations in terms of both function and appearance.  Over the past two decades a number of patients have received donor hand transplants, and while such procedures can significantly improve quality of life, they also expose recipients to the risks of life-long immunosuppressive therapy.  While the progenitor cells needed to regenerate all of the tissues that make up a limb could be provided by the potential recipient, what has been missing is the matrix or scaffold on which cells could grow into the appropriate tissues. The current study uses technology Ott discovered as a research fellow at the University of Minnesota, in which living cells are stripped from a donor organ with a detergent solution and the remaining matrix is then repopulated with progenitor cells appropriate to the specific organ.  His team and others at MGH and elsewhere have used this decellularisation technique to regenerate kidneys, livers, hearts and lungs from animal models, but this is the first reported use to engineer the more complex tissues of a bioartificial limb. The same decellularisation process used in the whole-organ studies – perfusing a detergent solution through the vascular system – was used to strip all cellular materials from forelimbs removed from deceased rats in a way that preserved the primary vasculature and nerve matrix.  After thorough removal of cellular debris – a process that took a week – what remained was the cell-free matrix that provides structure to all of a limb’s composite tissues.  At the same time, populations of muscle and vascular cells were being grown in culture. [caption id="attachment_21743" align="alignright" width="300"] A suspension of muscle progenitor cells is injected into the cell-free matrix of a decellularized rat limb, which provides shape and structure onto which regenerated tissue can grow. (Bernhard Jank, MD, Ott Laboratory, Massachusetts General Hospital Center for Regenerative Medicine)[/caption] The research team then cultured the forelimb matrix in a bioreactor, within which vascular cells were injected into the limb’s main artery to regenerate veins and arteries.  Muscle progenitors were injected directly into the matrix sheaths that define the position of each muscle.  After five days in culture, electrical stimulation was applied to the potential limb graft to further promote muscle formation, and after two weeks, the grafts were removed from the bioreactor.  Analysis of the bioartificial limbs confirmed the presence of vascular cells along blood vessel walls and muscle cells aligned into appropriate fibres throughout the muscle matrix. Functional testing of the isolated limbs showed that electrical stimulation of muscle fibres caused them to contract with a strength 80 per cent of what would be seen in new-born animals.  The vascular systems of bioengineered forelimbs transplanted into recipient animals quickly filled with blood which continued to circulate, and electrical stimulation of muscles within transplanted grafts flexed the wrists and digital joints of the animals’ paws. The research team also successfully decellularised baboon forearms to confirm the feasibility of using this approach on the scale that would be required for human patients. [caption id="attachment_21745" align="alignright" width="212"] After vascular and muscle progenitors have been introduced into a decellularized rat limb, it is suspended in a bioreactor, which provides a nutrient solution and electrical stimulation to support and promote the growth of new tissues. (Bernhard Jank, MD, Ott Laboratory, Massachusetts General Hospital Center for Regenerative Medicine)[/caption] Ott notes that, while regrowing nerves within a limb graft and reintegrating them into a recipient’s nervous system is one of the next challenges that needs to be faced, the experience of patients who have received hand transplants is promising.  “In clinical limb transplantation, nerves do grow back into the graft, enabling both motion and sensation, and we have learned that this process is largely guided by the nerve matrix within the graft. We hope in future work to show that the same will apply to bioartificial grafts.  Additional next steps will be replicating our success in muscle regeneration with human cells and expanding that to other tissue types, such as bone, cartilage and connective tissue.”